林秀坤：中科院研究員、基因工程專家

一， 個(gè)人簡(jiǎn)介

林秀坤，男，研究員，博士生導(dǎo)師，中國(guó)農(nóng)業(yè)科學(xué)院二級(jí)人才，中國(guó)科學(xué)院百人計(jì)劃。

二，主要兼職

中國(guó)藥學(xué)會(huì)生化與生物技術(shù)藥物委員會(huì)委員；中國(guó)抗癌協(xié)會(huì)抗腫瘤藥物專業(yè)委員會(huì)副主委；美國(guó)耶魯大學(xué)兼職教授；中國(guó)科學(xué)院研究員。

三，承擔(dān)課題

1、 哺乳動(dòng)物的性別控制機(jī)理研究，農(nóng)科院人才課題

2、 牛SRY基因的表達(dá)調(diào)控與體外功能鑒定，國(guó)家自然科學(xué)基金課題

3、 牛重要性別控制基因的調(diào)控網(wǎng)絡(luò)解析，國(guó)家863專題課題

4、 胸苷酸合成酶的表達(dá)調(diào)控，國(guó)家自然科學(xué)基金課題

5、 抗腫瘤多肽的研制與開發(fā)，國(guó)家863重點(diǎn)課題

四，主要著作與論文

（一）著作

1．《Fluoropyrimidines in Cancer Chemotherapy》,2002, 由美國(guó)Y.M. Rustum Human Press Inc. 出版；

2．《抗腫瘤藥物的研制與開發(fā)》，甄永蘇主編，2004，化學(xué)工業(yè)出版社

（二）主要論文

1． Zheng L., Wang Z., Niu R., Hu C and Lin X**., A novel peptide from shark cartilage with potent antiangiogenesis activity. Cancer Biol. Therapy, 2007,6（5）:775-780.

2． Wang Z, Zheng L, Yang S, Niu R, Chu E, Lin**. X. N-acetylchitooligosaccharide is a potent angiogenic inhibitor both in vivo and in vitro. Biochemical and biophysical research communications. 2007, 357(1): 26-31

3． Thakur A, Rahman K, Wu J, Bollig A, Biliran H, Lin X, Nassar H, Grignon DJ, Sarkar FH, Liao JD. Aberrant expression of X-linked genes RbAp46, Rsk4, and Cldn2 in breast cancer. Mol Cancer Res. 2007, 5(2):171-81.

4． Du C, Niu R, Zhang P., Chu E and Lin X**. Sequence analysis and functional study of thymidylate synthase from zebrafish, J Biochem, 2006,139, 913–920

5． Tai N, Schmitz JC, and Lin X et al, Translational autoregulation of thymidylate synthase and dihydrofolate reductase, Fron Bio Sci. 2004, 9:2521-2526

6． Lin X., Liu J., Chu E., et al, Role of cysteine amino acid residues on the RNA binding activity of human thymidylate synthase. Nucleic Acid Res. 2003, 31: 4882-4891

7． Yan Song,Niu Rongli,Wang Zheng,Lin Xiukun**.In vitro selected peptides bind with thymidylate synthase mRNA and inhibit its translation.Science in China Series C:life Sciences:2007.50(5):630-636.

8． Zhao J., Du W., Lin Xiukun**. Study on The RNA Interference of Sry in Mouse Embryos Progress of Biochem. Biophys. 2006.(9)：890-894

9. 張麗，林秀坤**等. 受精環(huán)境對(duì)哺乳動(dòng)物性別形成的影響. 遺傳. 2006, (1):17-21

10. 吳寧，廖冰， 杜衛(wèi)華，林秀坤* “siRNA介導(dǎo)Sry基因沉默對(duì)小鼠胚胎性別決定基因表達(dá)的影響”遺傳. 2008 2

11 chimitz JC, Lin X, Chu E. et al. Molecular mechanisms regulating the expression of thymidylate synthase. Fluoropyrimidines in cancer therapy. 2002, pp67-82, edited by Y.M. Rustum Humana Press Inc., Totowa, NJ.

12 iu J., Lin X, Chu E. et al. Thymidylate synthase as a translational regulation of cellular gene expression. Biochim. Biophys. Acta 2002, 1587:174-182.

13 Lin X., Liu J. and Chu E. et al. Effect of cysteine residues on RNA binding activity of thymidylate synthase. Proc. Am. Association for Cancer Res. 2002, 43:816

14．Schimitz JC, Lin X, Chu E. et al. Translational regulation as a novel mechanism for development of cellular drug resistance. Cancer and Metastasis Reviews. 2001, 20:33-41

15．Lin X., Parsels L.A., and Chu E. et al. Characterization of a cis-acting regulatory element in the protein coding region of thymidylate synthase mRNA. Nucleic Acid Res.2000, 28:1381-1389.

16．Lin X., Mizunuma N. and Chu E. et al. In vitro selection of an RNA sequence that interacts with high affinity with thymidylate synthase. Nucleic Acid Res.2000, 28:4266-4274.

17、裴杰 杜衛(wèi)華 劉小林1 柳向軍 朱化彬 趙金紅 林秀坤. 牛SRY 蛋白表達(dá)純化與體外功能鑒定，動(dòng)物學(xué)報(bào). 52 (6) :1082 - 1087 , 2006

18、裴杰, 劉小林, 杜衛(wèi)華, 林秀坤. 中國(guó)荷斯坦牛SRY 基因編碼區(qū)的克隆與原核表達(dá)

西北農(nóng)林科技大學(xué)學(xué)報(bào)(自然科學(xué)版) 2006, 第34 卷第7 期

19、張麗，杜衛(wèi)華， 陳宏，趙金紅，裴杰，林秀坤 “生殖激素對(duì)小鼠胚胎性別影響的研究” 分子細(xì)胞生物學(xué)報(bào) 2006,39(6) 573-576,577