Lab semen-Processing

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 Lab Processing

1. Clean, dry, no UV light. *
2. Lab tech clean – wash hands – lab coat on *.
3. Supplies in plastic containers *
4. Good H2O(Distilled water)for making extender ( recommended is Baxter bottled H20) *
5. Make up extender properly – temp – extender made up at least 1 hour prior to use (for it to balance and balance – proper warmer. * )
6. Cups warmed & kept clean *
7. Proper recording of - boars - collected every 7 to 10 days – proper semen extending records – morphology records done on each boar every month. *
8. Is semen extended within 3 to 5 min after brought to lab?  *
9. Calibration of sperm Q done regularly and recorded. * DAILY
10. Check microcruvetts to make sure they read 0 - *
11. Semen extended properly *

–        Swirl container before taking samples and temp check *

–        Using clean pipette for each sample *

–        Warm cover slides, sample covered immediately, looked at promptly. *

–        Temp. check of semen and extender each time *

–        Extender into semen slowly poured up against side of container. *

–        Semen checked for motility again after extending *

–        Dispensing tube applied properly * USING BAG

12. Packaging of semen

   –     Mix semen before packaging *

–      Pour into side of tubes reducing foam *

–      Mix semen after pouring every 6 to 10 does *

–      Fill tubes to 80ml in proper tube color for genetic line *

–      Seal promptly * YES THEY MAKE SURE CAPS ARE TIGHT.

13. Labeling done properly  - Legible  - ID – Date
14. All semen out of UV light * YES
15. Storage cooling unit -  does it have a check list for temperatures – is it clean –  Does anyone look at this list to ensure compliance?  *
16. Are doses cooled promptly that are not being used that day? *
17. Semen not stacked when cooling – cool to 17C *